ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
10.3
STAINING AND COVERSLIPPING
Table 10.1: Hologic Staining Protocol
*Time may vary with laboratory preference.
Solution Time*
1. 70% Reagent Alcohol 1 minute with agitation
2. 50% Reagent Alcohol 1 minute with agitation
3. Distilled H
2
O (dH
2
O) 1 minute with agitation
4. Richard-Allan Hematoxylin I 30 seconds with agitation
5. Distilled H
2
O (dH
2
O) 15 seconds with agitation
6. Distilled H
2
O (dH
2
O) 15 seconds with agitation
7. Clarifier (0.025% glacial acetic acid) 30 seconds with agitation
8. Distilled H
2
O (dH
2
O)) 30 seconds with agitation
9. Bluing Reagent (10mg LiCarb/1L) 30 seconds with agitation
10. 50% Reagent Alcohol 30 seconds with agitation
11. 95% Reagent Alcohol 30 seconds with agitation
12. Richard-Allan Cytology Stain 1 minute with agitation
13. 95% Reagent Alcohol 30 seconds with agitation
14. 95% Reagent Alcohol 30 seconds with agitation
15. 100% Reagent Alcohol 30 seconds with agitation
16. 100% Reagent Alcohol 30 seconds with agitation
17. 100% Reagent Alcohol 30 seconds with agitation
18. Xylene 1 minute with agitation
19. Xylene 1 minute with agitation
20. Xylene 3 minutes with agitation
21. Mount per your laboratory’s protocol
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