Hologic ThinPrep 5000 - Limitations and Contraindications

INTRODUCTION
1.4 ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
1
pressure behind the filter membrane. The slide is delivered to a staining rack immersed in an alcohol
fixative bath.
(For ancillary testing preparation and instructions, please refer to “OPTIONAL INSTRUCTIONS
FOR ANCILLARY TESTING” on page 7.37.)
Figure 1-2 The ThinPrep Sample Preparation Process
Limitations
• Gynecologic samples collected for preparation using the ThinPrep 5000 system should be
collected using a broom-type cervical collection device or endocervical brush/plastic spatula
combination collection device. Refer to the instructions provided with the collection device
for warnings, contraindications, and limitations associated with specimen collection.
• Preparation of microscope slides using the ThinPrep 5000 system should be performed only
by personnel who have been trained by Hologic or by organizations or individuals
designated by Hologic.
• Evaluation of microscope slides produced with the ThinPrep 5000 system should be
performed only by cytotechnologists and pathologists who have been trained to evaluate
Dispersion
The sample vial is
rotated, creating
currents in the fluid
that are strong enough
to separate debris and
disperse mucus, but
gentle enough to have
no adverse effect on
cell appearance.
Cell Collection
A gentle vacuum is created
within the ThinPrep Pap test
filter, which collects cells on the
exterior surface of the
membrane. Cell collection is
controlled by the
ThinPrep
™
5000 processor’s
software that monitors the rate
of flow through the ThinPrep
Pap test filter.
Cell Transfer
After the cells are collected on
the membrane, the ThinPrep Pap
test filter is inverted and gently
pressed against the ThinPrep
microscope slide. Natural
attraction and slight positive air
pressure cause the cells to adhere
to the ThinPrep microscope slide
resulting in an even distribution
of cells in a defined circular area.