Shimadzu SPD-M20A - Light Source Spectrum; Saturation Level Check; Absorbance Accuracy Calibration and Check; Required Items; Absorbance Accuracy Linearity Calibration Procedure

Chapter 6: Maintenance and Adjustments
SPD-M20A Service Manual
59
Spectra Bandwidth
The 253.7 nm wavelength spectral bandwidth of the Hg lamp should be 1.4 nm max. with
the 1.2 nm slit, and 10 nm with the 8 nm slit.
Wavelength Standard
True Value
Bandwidth Measurement Value
Standard Value
Hg 253.7 nm
(1.2 nm)
－
< 1.4 nm
Hg 253.7 nm
(8 nm)
－
< 10 nm
< Light Source Spectrum / Saturation Level Check >
Procedure
Install a water-filled flow cell, and take the following light source spectral data.
Perform this with the 1.2 nm slit and 8 nm slit, using the PDA Utility "Optical Check".
1.2 nm Slit
Item
Y-axis [V/FS]
X-Axis [nm]
Standard Value
Dark
1
190 ~ 800
< 0.8 V
D2
10
190 ~ 800
> 7 V
Saturation level*
10
190 ~ 800
> 9 V
Semi-micro cell (D2)
10
190 ~ 800
> T1/ 32
D2 + W
10
190 ~ 800
> 7 V
W
10
190 ~ 800
> 7 V
8 nm Slit
Item
Y-axis [V/FS]
X-Axis [nm]
Standard Value
D2
10
190 ~ 800
> 7 V
D2 + W
10
190 ~ 800
> 7 V
W
10
190 ~ 800
> 7 V
* When performing the saturation level measurement, remove the flow cell.
1) Install a water-filled semi-micro cell (P/N 228-34278-91), graph the D2 lamp spectral
data, and read the maximum value of P(V).
2) Assuming T1 (ms) is the exposure time setting, verify that P satisfies the following
expression.
P > (T1/ 32)
6.6 Absorbance Accuracy Calibration and Check
< Required Items >
Name
P/N
Qty
Photometric accuracy cell
228-35011-91
1
Standard filter
228-40251
1
JCRM230A (with JQA Certificate)
< Absorbance Accuracy Linearity Calibration Procedure >
1) Open the PDA Utility "Absorbance Accuracy Check / Correction".
2) Select [Settings]-->[Check Conditions Settings], and perform the settings shown in
the table below.