Shimadzu SPD-M20A - Absorbance Linearity Calibration (Stray Light Correction) and Check; Required Items and Reagents; Absorbance Linearity Calibration (Stray Light Correction) Procedure

Chapter 6: Maintenance and Adjustments
SPD-M20A Service Manual
62
6.7 Absorbance Linearity Calibration (Stray Light Correction)
and Check
< Required Items and Reagents >
Name
P/N
Remarks
Methanol for Liquid
Chromatography
Used to dilute 2 AU and 0.5 AU caffeine solutions.
4 AU caffeine solution
228-40480-03
Caffeine dissolved in LC methanol
(Concentration: 90 mg/L, Absorbance: approx. 4
AU)
2.0 AU caffeine/methanol
solution
Caffeine dissolved in LC methanol
(Concentration: 44 mg/L, Absorbance: approx. 2
AU)
*Record the weight of the caffeine actually
dissolved.
0.5 AU caffeine/methanol
solution
228-40480-01
2.0 AU caffeine/methanol solution is diluted to 1/4
concentration using LC methanol.
(Concentration: 11 mg/L, Absorbance: approx. 0.5
AU)
*Dilution method
Use a 250 mL measuring flask to measure out 2.0
AU caffeine/methanol solution into a 1 L
measuring flask, and add LC methanol to create a
total volume of 1 L. Measuring flasks used to
create 0.5 AU and 2.0 AU solutions should be
specialized for that purpose.
Balance
For weighing the caffeine.
Disposable syringes (2 mL)
4
Adaptors for disposable
syringes
Male nuts (PEEK), couplings, etc.
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