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does not separate in one cycle, the puller will read this line repeatedly, which is called
“Looping” and pull the glass in multiple stages.
If the program is appropriate for making a long microinjection needle or electrode
(Pull > 40 and Vel > 50), the glass will separate in one stage. If the program is intended
to pull a patch type pipette (Pull= 0 and Vel = 20-70), a one-line program, as exampled
below, will execute in many stages or “Loops” until the glass separates.
In this “Patch” program, Line 1
is asked to execute one time, but
the program will “Loop”
multiple times until the glass
separates. The expected number
of loops has been noted in the
text box.
To fine-tune the program, touch
the velocity value to highlight
(black square will appear) and
use the keypad or knob to edit
and enter a higher or lower
value.
Figure 2-12. Single line looped program.
As another example, this
“Patch” program, Line 1 will be
executed 3 times and then Line
2 will be executed 1 time.
Figure 2-13. Two-line program with first line looped.
2.6.1 Installing a Program
When pulling any type of pipette, it is best to first install a Cookbook program or a program
you know worked well on a P-97 or P-1000 using the same glass and filament. Always run a
new ramp test when installing a new program. If the program you install was established on
a different puller with a different filament and/or glass size, you cannot expect the program
to behave the same and make the same morphology of pipette. If your program parameters
are established randomly or a specific setting is too high or too low, the program will be
unstable and will not execute as expected. Please take note of the final pull report when
pulling pipettes to make sure your program has executed properly.
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)
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