Sutter Instrument P-1000 - Technical Tips for Pulling Micropipettes; Regulating the Time It Takes to Pull a Sharp Pipette; Heat

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4
be equal to the VELOCITY value that results with the glass separating after 2 loops. Set
your program VELOCITY, to a value midway between Y and Z. This value will be a very
stable VELOCITY value and will provide you with the most reproducible results.
6. The one line program just established may be sufficient for your application. However,
changes made in a one-line program are amplified throughout the cycle, potentially
producing gross changes in the pipette. If you need to make fine adjustments to the
pipette geometry, then you should use a multi-line program. The multi-line program is
based on the one line program just established. It is developed as follows:
7. Write your one-line, looping program out into an equivalent multi-line program with the
number of lines equal to the number of loops.
For example, a one line, 4-loop program with the following values can be written:
Line Loops HEAT PULL VELOCITY
DELAY
1 4
525
0 23 1
The above 1-line, 4-loop program could be written into an equivalent 4-line program,
as follows:
Line Loops HEAT PULL VELOCITY
DELAY
1 1
525
0 23 1
2 1
525
0 23 1
3 1
525
0 23 1
4 1
525
0 23 1
Now, you can make adjustments to the last or next to last line to fine-tune the program and
the resulting pipette.
Recommended changes to fine tune the multi-line program:
For larger diameter tips Decrease HEAT in last line.
For smaller diameter tips Increase or decrease VELOCITY in next to last line by 2 to 3
units, or increase/decrease VELOCITY in last line by 2 to 3
units, or add a small amount of PULL (10 to 20) to last line.
4.9 Technical Tips for Pulling Micropipettes
4.9.1 Regulating the Time it takes to pull a Sharp Pipette
4.9.1.1 HEAT
For 1.0mm O.D. tubing, if the pull takes longer than eight seconds and you are trying to pull
a fine micropipette, increase the HEAT. To do this in a methodical fashion, increase the
HEAT value in five unit increments, each time monitoring pull time until the pull takes
place in less than eight seconds.
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)