4
6. You can accept the ramp value (in which case the value gets installed into your program),
repeat the ramp test, or ignore it and go back.
7. Accept the ramp value and use a heat-setting equal to, or within five (5) units of, the
ramp test value. This heat setting will be a stable and safe heat to use—one that will not
create instability or damage the filament.
4.6.1 Heat Value Recommendations
For Trough filaments: Recommended starting value: Ramp + 5 units
Maximum program HEAT value(s) = Ramp value + 10%
For Box filaments: Recommended starting value: Ramp value = HEAT setting.
Maximum Program HEAT value(s) = Ramp value + 10%
4.7 Pulling Pipettes
4.7.1 Procedures
Prior to pulling a pipette for the first time, it is important to establish what HEAT value is
appropriate to melt your glass. Before executing a program for the first time, run the RAMP
TEST as previously described, and accept the Ramp value into the program. The HEAT
settings in the factory-installed programs are typically set at Ramp value. If your Ramp test
value differs from the factory Ramp value, adjust the HEAT in your programs to your Ramp
value.
Once you have adjusted the HEAT value relative to the Ramp value, pulling a pipette is very
straightforward. Try executing the factory installed programs with the sample glass to
become acquainted with the pulling process.
1. Load the glass into the puller as described previously in the FIRST TIME USE chapter.
2. Press
and then , to open Program 0.
3. Inspect the parameter values displayed for Program 0. Program 0 should display the
factory-installed values listed on the enclosed program sheet. Adjust the HEAT setting to
your Ramp value.
4. Press the < PULL > button. The heating
filament will turn on and the glass should
separate within 10 seconds. The display
will then report the number of heating
cycles and the total time that the heat was
turned on.
Figure 4-25. Pull Report.
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)
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