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1. GENERAL INFORMATION
1.1 Introduction
The P-1000 can fabricate pipettes for use in intracellular recording, patch clamping,
transferring (ICSI, ES Cells), microinjection, aspiration, and microperfusion. Realizing the
full potential of this instrument is dependent on a complete understanding of the way it
implements the pulling process. To this end, we urge that this manual be read in its entirety.
To aid in understanding the function of the instrument, sample programs are already loaded
in memory (Programs 0 through 5, as discussed in subsequent material).
The Model P-1000 Flaming/Brown Micropipette Puller combines a proven pulling technology
with programmability to produce a very versatile instrument. The pulling mechanism is
derived from the Flaming/Brown series of pullers, which have demonstrated the ability to
pull a complete range of pipette profiles. Added to this mechanism is the ability to program
different pulling sequences; thus, allowing ease of use for pulling a variety of pipette types on
one device.
The P-1000 is a ‘velocity sensing’ puller. This patented feature allows the puller to indirectly
sense the viscosity of the glass, giving the P-1000 the ability to pull pipettes from all glasses
except quartz. Even difficult to pull formulations, such as aluminosilicate glasses, are
handled with relative ease.
Throughout this manual reference will be made to the size of the glass tubing used to pull
micropipettes. The convention used here for describing the outside diameter (O.D.) and
inside diameter (I.D.) is as follows: O.D. x I.D. These dimensions will always be given in
millimeters (mm). See our catalog or visit
www.sutter.com to find a wide selection of glass
capillaries available for purchase.
1.2 Glass Capillary & Heating Filament Specifications
1.2.1 Glass Capillary
The P-1000 micropipette puller is designed for use with aluminosilicate, borosilicate or other
lower melting-point glass tubing or rod ranging from 0.6 to 3.0 mm in outer diameter. This
instrument does not pull quartz glass. Examples of the specific types and sizes of glass that
can be used with the P-1000 are listed in the Sutter Instrument Company catalogue that was
included with this instrument or can be viewed on Sutter Instrument’s web site at
www.sutter.com. Any glass with comparable technical specifications can be used with the P-
1000.
1.2.2 Heating Filament
The type and size of glass that you choose may require a Heating Filament other than the
one installed in your puller at the factory. Please refer to the Heating Filament section of
this manual to determine the appropriate style and size of filament necessary for pulling the
specific glass you would like to use. This instrument is designed to accommodate any of the
Sutter Instrument Trough-type or Box-type filaments that are shown in the Sutter
Instrument catalogue. This selection of replacement filaments can also be viewed on Sutter
Instrument’s web site at
www.sutter.com.
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)
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