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1. The filament may not be centered over the air jet. Follow the procedure “Testing the
position” in the Heating filament replacement section of the Maintenance chapter.
2. If the filament is correctly centered, then the tension in the two cables that transmit the
pulling force from the solenoid to the puller bars might not be equal. To check the tension
and adjust if necessary, first contact Sutter Instrument to discuss the details and then
follow the procedure “Pulley Adjustment” in the Maintenance chapter.
6.1.9 Problem: The shape and resistance of the pipette changes from pull to pull.
1. In most cases, this is due to not using the midpoint velocity when making a patch pipette
or the program is unstable. See the Pipette Cookbook for recommended parameter
settings for your application.
2. If the problem persists, then run the ramp test several times. If possible, use one long
piece of glass and move the glass over after each ramp test. If the ramp values are +/- 4
units or less the problem may be with the glass. If the values are greater than +/- 4 units
call Sutter Instruments.
6.2 System Operation/Function Problems
The P-1000 has many built-in automated test capabilities. When the system starts up, the
hardware is tested for normal operation and error messages are generated upon detection of
a failure. Additionally, automated testing occurs before each pull. Finally, a diagnostics menu
screen is provided, allowing for diagnostic testing on demand.
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)
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