Sutter Instrument P-1000 - 4.2.3 Pull Cycle; 4.2.4 Start Up; 4.8.2.1 HEAT; 4.8.2.2 PULL Strength

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the glass, which in turn is a function of the glass temperature. The
adjustable velocity allows for a selection of a precise glass
temperature as the trip point for the hard pull. Useful values for
velocity range from 10 to 150 with lower values being used for patch
and injection pipettes and higher values for micropipettes. See the
Programs section for a discussion of the significance of
VELOCITY=0.
TIME
(Range 0-500)
TIME is one of two available modes of cooling and controls the length
of time the cooling air is active. If VEL>0 then one unit of TIME
represents 1/2ms. If VEL=0 (when in fire-polish mode), then one
unit of TIME represents 10ms. See the Programs section for a
discussion of the significance of TIME=0.
Delay Mode
not checked
DELAY
(Range 0-500)
DELAY is a cooling mode that controls the delay time between when
the heat turns off and when the hard pull is activated. The air is
automatically turned on for 300ms. The higher the DELAY value, the
cooler the glass will be when the hard pull is executed. Thus,
increasing the DELAY results with decreased taper length and
increased tip diameter. If VEL>0 then one unit of DELAY represents
1 (one) ms. If VEL=0 (when using fire-polish mode), then one unit
represents 10 (ten) ms. See the Programs section for a discussion of
the significance of DELAY=0.
Delay Mode
checked
4.2 Programs
4.2.1 Program Structure
The resulting size and shape of a micropipette made using the P-1000 is determined by the
parameter values that are programmed by the user. Up to 100 separate programs can be
installed and saved. Each program is structured as follows:
Program Consists of one or more Lines, each of which represents a pull cycle.
When a program is run, all lines within the program are sequentially
executed, beginning with Line 1. As a line is executed, the capillary glass
inserted in the instrument is “pulled.” A program can consist of up to 4
lines, and each line can be repeated up to 4 times with a maximum total
of 10 pull cycles for the program.
Cycle A Cycle consists of six programmable parameters: Line, Heat, Pull,
Velocity, either Time or Delay, and Pressure. A Cycle is equivalent to one
line of Program code. Each Cycle can be executed up to four times.
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)