20
Figure 2-17. Delay mode of air-cooling.
An example of the DELAY mode of
air-cooling is shown in the following
figure.
The Delay Mode is recommended when additional cooling and more control over the viscosity
of glass is required. The Delay Mode is often used for the following applications:
Patch Pipettes using Thick Walled Glass
Short microinjection needles for Adherent Cell Microinjection
Pronuclear Injection pipettes
2.6.7 Jaw Temperature
The jaw temperature is displayed at
the end of pull and can be used to
help define the best pulling
conditions for making your pipettes.
Therefore, for example, you might
find that your pipettes are best
formed when the jaw temperature is
between 52 and 58 degrees
Centigrade. If the jaw temperature
exceeds 125 degrees Centigrade, a
warning will be displayed and the
puller will shut down. This “shut
down” protects the puller and
filament assembly from damage due
to overheating. When the jaws have
sufficiently cooled, you can proceed
to pull pipettes.
Figure 2-18. Jaw temperature.
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)
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