Agilent 1260 Infinity DAD and MWD User Manual 131
How to optimize the Detector
6
Optimizing Selectivity
Ratio Qualifiers for Selective Detection of Compound Classes
Ratio qualifiers can be used where, in a complex sample, only one particular
class needs to be analyzed — a parent drug and its metabolites in a biological
sample, for example. Another example is the selective analysis of derivatives
after pre- or post-column derivatization. Specifying a signal ratio that is
typical for the sample class is one way of selectively plotting only those peaks
that are of interest. The signal output remains at zero so long as the ratio is out
of the user-specified ratio range. When the ratio falls within the range, the
signal output corresponds to the normal absorbance, giving single, clear peaks
on a flat baseline. An example is shown in Figure 60 on page 131 and
Figure 61 on page 132.
Figure 60 Wavelength Selection for Ratio Qualifiers
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