40 Agilent 6500 Series Q-TOF LC/MS Maintenance Guide
2Maintenance
To prepare the reference mass solution
To prepare the reference mass solution
The dilutions in this topic are used for installation and check-out. You may
need to adjust the dilution for your own analysis.
Before you begin, check that you have:
• The G1969-85001 ES-TOF Reference Mass Solution Kit, which contains two
ampoules each (2.2 mL/ampoule) of the following reference ions:
• 100 mM (millimolar) ammonium trifluoroacetate in 90:10
acetonitrile:water. This solution is abbreviated 100 mM TFANH4.
• 5 mM (millimolar) purine in 90:10 acetonitrile:water
• 2.5 mM (millimolar) hexakis(1H, 1H, 3H-tetrafluoropropoxy)phosphazine
in 90:10 acetonitrile:water. This solution is abbreviated 2.5 mM HP-0921.
• Acetonitrile UV - B&J Brand High Purity Solvent for HPLC, Gas
Chromatography, Pesticide Residue Analysis and Spectrophotometry.
(Material number: 10071618, Cat. number 015-4).
• Nanopure D.I. Water (18 megaΩ-cm, organic-free)
The reference solution provides internal reference masses for reference mass
correction in positive and negative ion modes of operation. For the purposes
of mass accuracy performance verification, make the following reference mass
solution.
1 Put on protective gloves.
2 Using a graduated cylinder, pour 950 mL of acetonitrile and 50 mL of water
into the 1-liter Nalgene bottle (p/n 9301-6460) supplied in the Q-TOF LC/MS
system ship kit.
3 Using a pipettor, add the following amounts of the individual calibrants to
the 1-liter Nalgene bottle containing the acetonitrile/water solution made in
step 2.
Table 6
Dual ESI Agilent Jet Stream ESI
TFANH
4
0.5 mL
Purine 1.0 mL 0.4 mL
HP-0921 0.45 mL 1.0 mL
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