70 Agilent 6500 Series Q-TOF LC/MS Maintenance Guide
2Maintenance
To flush the nebulizer monthly
To flush the nebulizer monthly
When required Monthly or as needed at the end of each shift (or more often) to flush traces of
samples and buffers out of the tubing, valves and nebulizer
Tools required • HPLC Flushing Solvent
• Cyclohexane, HPLC-grade or better
• Acetonitrile, HPLC grade or better
• Alternatively if available: isooctane, HPLC-grade or better
Parts required None
1 Make sure HPLC flushing, cyclohexane, and acetonitrile are three of the
solvents installed in your liquid chromatograph.
2 Pump HPLC flushing solvent for 10 minutes at 5 mL/minute.
3 Switch to cyclohexane, and pump for 10 minutes at 5 mL/minute.
4 Pump this mixture through the nebulizer for 3 minutes.
5 Prepare enough acetonitrile and flush overnight.
This is a good general-purpose flushing mixture but you may need to adjust it based on the
solvents, samples and buffers you are using. For example, a mixture of 50% acetonitrile and
50% water works well for removing salts.
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