44 Agilent 6500 Series Q-TOF LC/MS Maintenance Guide
2Maintenance
To fill a tuning mix or reference mix bottle
To fill a tuning mix or reference mix bottle
When required As needed.
Tools required None
Parts required • APCI-L Tuning Mix (p/n G1969-85010)
• ESI-L Tuning Mix (p/n G1969-85000)
• MMI-L Tuning Mix (p/n G1969-85020)
• Reference Mix (created from G1969-85001 Reference Mass Kit)
Instruments with Agilent Jet Stream Technology uses diluted ESI-L Tuning
Mix. See “To prepare tuning mix” on page 35.
1 Turn the bottle to be refilled clockwise until it can be removed from the
fixed bottle cap.
2 Refill the bottle with the appropriate tuning mix.
3 Put the intake tube into the refilled bottle as you lift the bottle into position.
4 Attach the calibrant bottle onto the fixed bottle cap. Turn the bottle
counterclockwise to tighten.
Tighten the bottle by hand. Do not overtighten it. The bottle only needs to be snug.
Record this procedure in the Maintenance Logbook.
The tuning mixes are not interchangeable, even though they will give similar mass spectra.
Failure to change the tuning mix when the source or inlet assembly is changed can result in
miscalibration of the instrument and erroneous mass assignments.