Agilent 6500 Series Q-TOF LC/MS Maintenance Guide 35
Maintenance 2
To prepare tuning mix
To prepare tuning mix
For many instruments, the standard tuning mix will produce correct results.
However, in some instances, a dilution is required.
Dilute the tuning mix if:
• You get error messages that instruct you to dilute the calibrant.
• The tune report shows abundances greater than 750,000 counts. You
need to dilute until the largest abundance is below 750,000 counts for a
mass axis calibration. Typical tune mass abundances are in the range of
50,000 to 650,000 counts.
Do not dilute the tuning mix if:
• Your source is an APPI or APCI.
Before you begin, make sure you have:
• G1969-85000 ESI-L Low Concentration Tuning Mix (for Dual ESI or ESI
with Jet Stream Technology)
• G1969-85020 MMI-L Low Concentration Tuning Mix (for multimode
source)
• G1969-85003 Biopolymer Reference Mass Kit, which contains the
HP-0321 solution (for ESI with Jet Stream Technology)
• HPLC-grade acetonitrile. Acetonitrile must be suitable for HPLC,
spectrophotometry, and pesticide residue analysis.
• Water, 18 MΩ-cm resistivity or better and free of organics.
• Glass CDS bottle (p/n 9300-2576) and cap (p/n 9300-2575)
The materials shipped with the instrument are of sufficient purity for this
purpose.
These instructions will make 100 mL of diluted tuning mix.
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