Agilent Technologies 6500 Series - Page 36

36 Agilent 6500 Series Q-TOF LC/MS Maintenance Guide
2Maintenance
To prepare tuning mix
1 Add the components in the table below, in the order listed, to a clean CDS
bottle. Refer to the table that is appropriate for your source or inlet
assembly.
Make sure you add the components in the order listed below to avoid
precipitation of any components of the tuning mix.
2 Mix the contents thoroughly and install the bottle on the instrument.
3 Change to the Tune context, then:
a Open the reference B valve.
Table 2 ESI with Jet Stream Technology
Source Positive Mode Negative Mode
Undiluted Tuning Mix 10 ml ESI-L 2.5 mL ESI-L
Acetonitrile 88.5 mL 95.6 mL
Water 1.5 mL 1.9 mL
0.1 mM HP-0321 (included in
the Biopolymer Reference
Mass Kit)
5 µL N/A
Table 3 Dual ESI
Components Positive Mode Negative Mode
Undiluted Tuning Mix 25 mL ESI-L 25 mL ESI-L
Acetonitrile 71.25 mL 71.25 mL
Water 3.75 mL 3.75 mL
Table 4 Multimode
Components Positive Mode Negative Mode
Undiluted Tuning Mix 25 mL MMI-L 25 mL MMI-L
Acetonitrile 71.25 mL 71.25 mL
Water 3.75 mL 3.75 mL