BD FACSymphony A5 SE - Page 116

starting 24
troubleshooting 104
D
DAPI, cleaning after using 37
data
analyzing 84, 87
gating 87
recording 84-85
debris, excessive 108
delay, laser 94
detector arrays 20
detector parameters 49
detectors 21
detergent solution 37
digital data 12
Diva software See software 8
droplet containment system 19
E
electronics
troubleshooting 109
event rate
erratic 107
high 107
low 107
zero 105-106
excessive debris 108
experiments
creating 74
immunophenotyping 84
sample optimization 74
specifying parameters 74
F
FACSFlow sheath fluid [f] 113
FACSFlow solution[f] 27
Falcon tubes 104
filters, sheath
attaching new 42
replacing 41
fixed-alignment lasers 12
116 BD FACSymphony™ A5 SE User's Guide
flow cell
draining 31
flow rate control buttons 16
fluid control buttons
PRIME 15
RUN 15
STANDBY 15
fluidics
description 15
flow rate control 16
flushing system 38
priming 31
removing air bubbles 28
run mode 15
sample injection port (SIP) 17-18
fluidics controls, cytometer 14
fluidics interconnect 29
fluorescence signal, none 106
flushing the system 38
G
gating data 87
global worksheets
analyzing data 87
creating 84
previewing data 84, 91
H
hazard symbol definitions 8
High Throughput Sampler (HTS) 18
I
immunophenotyping
analysis 87
experiment 84
L
laser channels 48
laser delay
about 94
optimizing 95