PowerWave Operator’s Manual 4-3
♦ Linearity of the Optical Density readings are confirmed by
default if the wavelength readings are accurate.
♦ Alignment of the plate carrier and standard microplate s
are confirmed by the four corner positional accuracy check.
♦ Channel-to-channel variation can be tested by completing
the turnaround test. This tests the readers ability to read
samples accurately in different plate positions.
♦ Wavelength setting accuracy. To check the accuracy of
wavelength settings, use the special Calibrator Test Plate,
Part Number 7260522. The Calibration Test Plate provides a
multiband calibration filter in location C6. The filter is
Didymium glass V10. The filter should be scanned between
580 and 590 nm in 1 nm increments using KC4. For
PowerWave 340, or if KC4 is not available, select six
wavelengths at 1 nm increments near the expected peak.
The wavelength of the maximum absorbance should be
compared with the wavelength written on the sheet
supplied with the calibration plate.
PEAK
WAVELENGTH (nm)
80
700600500400300200
4.5
4
3.5
3
2.5
2
1.5
1
0.5
0
TYPICAL ABSORBANCE SPECTRUM:
DIDIMIUM GLASS V-10
W AVELEN G TH O F PEA K IN THE
INTERVAL BETWEEN 580nm AND 590nm
MULTIBAND CALIBRATION FILTER
IN LO C ATIO N C 6
To Next Page
Loading...
Need help?
General
