Biotek PowerWave - Kinetic Data Analysis Selection

3-14 Operation
• Use SOFT KEY 1 to select low-speed (17 Hz) shaking.
• Use SOFT KEY 2 to select medium-speed (18 Hz) shaking.
• Use SOFT KEY 3 to select high-speed (19 Hz) shaking.
• Use SOFT KEY 4 to select variable-speed shaking ( 1 second of each
speed repeated).
KINETIC DATA ANALYSIS SELECTION
Use this screen (Figure 3-18) to select the type of kinetic analysis.
K
I N E T
I
C A N A L Y S I S : R - S Q R
R A T E R - S Q R O N S E T
Figure 3-18: Kinetic Data Analysis selection screen
• Use SOFT KEY 1 to select the kinetic rate calculation. This method
will apply a linear fit to calculate the maximum slope in mOD/min.
based on the number of kinetic points specified.
• Use SOFT KEY 2 to select the R-squared rate calculation. This
method will calculate the R-squared value at the maximum slope,
based on the linear curve fit and the number of kinetic points
specified.
• Use SOFT KEY 3 to select the time calculation, which will calculate
the time for each well to reach the onset optical density.