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Chapter 4
Performance Verification
This chapter includes instructions
for using the PowerWave’s utilities
to achieve performance verification.
Discussed in this chapter are running the
System tests, and using the Calibration Test Plate.
This chapter also discusses Linearity,
Accuracy and Repeatability tests.
Achieving Maximum Performance
Here are some recommendations to help you achieve maximum performance.
• The microplate should be perfectly clean and free of dust or bottom
scratches. Use new microplates from sealed packages. Do not allow
dust to settle on the surface of the solution; use microplate covers
when not reading the plate.
• Although the PowerWave works with all flat, U-bottom, and V-
bottom microplates, it performs best with optically clear, flat-
bottomed wells. Non-uniformity in the optical density of the well
bottoms can cause loss of accuracy, especially with U- and V-bottom
polyvinyl microplates. Check for this by reading an empty
microplate. Dual wavelength readings can eliminate this problem,
or bring the variation in density readings to within acceptable limits
for most measurements.
• Inaccuracy in pipetting has a large effect on measurements,
especially if smaller volumes of liquid are used. For best results, use
at least 100 microliters.
• The inclination of the meniscus can cause loss of accuracy in some
solutions, especially with small volumes. Agitate the microplate
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