4-2 Performance Verification
before reading to help bring this problem within acceptable limits.
Use Tween 20, if possible, (or some other wetting agent) to
normalize the meniscus.
Some solutions develop menisci over a period of several minutes.
This effect varies with the brand of microplate. As the center of the
meniscus drops and shortens the light path, the density readings
change. The meniscus shape will stabilize over time.
Installation and Operational Qualification Procedures
Tests outlined in this section may be utilized to confirm initial and ongoing
performance of PowerWave.
Your PowerWave reader was fully tested at Bio-Tek prior to shipment and
should operate properly upon initial setup. If it is suspected that problems
may have occurred during shipment, if the device was reshipped by you, or
if regulatory requirements dictate that Installation Qualification Testing is
necessary, the following tests should be performed. After the initial
confirmation of operation, this testing should be performed annually.
• System Self Test: Verifies the proper gains, bulb operation, and
optional incubator functionality. There will be a document print-
out that shows that the testing was done.
• Wet (dye) testing confirms the linearity and alignment.
• Calibration Test Plate:
♦ Accuracy of the Optical Density readings -The comparison
of the wavelength readings with those given with the
Calibration Test Plate insert will confirm the accuracy of the
wavelengths.
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