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Biotek PowerWave - Defining the Curve-Fit; Curve-Fit

Biotek PowerWave
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3-52 Operation
Defining the Curve-Fit
The CURVE entry screens allow editing and entry of:
Curve-Fit Type
Editing of Outliers
Axis Identification
Extrapolation of Unknowns
These screens are displayed on the PowerWave in the order in which they
appear in the assay. If a closed variable (i.e., an element of the assay
definition that the user cannot access or modify) is being used in the assay,
the entry screen is omitted.
CURVE-FIT
The Curve-Fit screen (Figure 3-60) allows the user to select the curve-fit
method that will be applied to the assay. Any previously defined curve-fit
type appears on the top line of the display, and available options on the
second line.
The Curve-Fit screen has three sub-menu screens. Each sub-menu screen
provides different curve-fit options for selection. These options include C-
Spline, Linear, Quadratic, Cubic, 4-P, 2-P (Logit/Log) PT to PT (point to
point), and None.
Linear curve fit: A simple best fit straight line is plotted using the
values of the standards.
Quadratic or “Quad” curve fit: A curve fit which uses the
Quadratic equation “ax
2
+bx +c=y” to plot the standards values.
Utilizing this curve, any data point for a standard that deviates from
the ideal value will not affect the entire curve.

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