PowerWave Operator’s Manual 3-9
SINGLE OR DUAL WAVELENGTH
The Wavelength selection screen (Figure 3-9) allows the user to select
SINGLE or DUAL wavelength for the assay.
If single wavelength is chosen, the reader measures the optical density of
each well with a single wavelength.
If dual wavelength is chosen, each well is read twice, each time with a
different wavelength. The microplate is not removed from the reading
chamber between the two measurements. The final reported optical density
is the difference between the two readings.
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NOTE: Dual wavelength readings significantly reduce optical interference caused by
scratches or fingerprints which absorb light equally at both wavelengths. Choose a REF
filter that is far from the MEAS filter in the spectrum for best results.
Any previously defined wavelength appears on the top line of the display,
and the options on the second.
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Figure 3-9: Specifying the Wavelength
• Press SOFT KEY 1 to select SINGLE wavelength. The top line of the
display is updated to reflect the selection.
• Press SOFT KEY 2 to select DUAL wavelength. The top line of the
display is updated to reflect the selection.
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