3-44 Operation
VALIDATION FORMULA EXAMPLES
• Blank Validation: An assay protocol states that the blank wells on a
plate should have an OD of less than 0.050. The formula is entered
on the reader as a Blank Validation Formula:
BLK < 0.050.
• Negative Control Validation: An assay protocol states that the
Negative Control wells must have an OD of less than 0.100. The
formula is entered as a Control Validation Formula:
NC < 0.100.
• Positive Control Validation: An assay protocol states that the
Positive Control wells must have an OD higher than 1.000, but less
than 2.500. Two Control Validation Formulas can be entered:
PC > 1.000
and
PC < 2.500.
Or, one formula can be used if the formula is 24 characters or less:
PC>1.000ANDPC<2.500.
• Assay Validation: An assay protocol states that in order for an
assay to be valid, the mean of the Negative Control well OD’s must
be less than 0.100. The Assay Validation formula that should be
entered:
NC;x < 0.100 (the map identifier NC;x indicates the
mean of the NC’s)
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