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Biotek PowerWave - Transformations; Transformation Formula Definition

Biotek PowerWave
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PowerWave Operator’s Manual 3-47
TRANSFORMATIONS
Transformation formulas change the absorbance data of all wells defined in
the Map, to another format, in preparation for further data reduction. Simple
transformations can change the OD values of all wells on the plate to some
other value, as described in Transformation Formula Definition below.
TRANSFORMATION FORMULA DEFINITION
From the assay Define Menu, press the arrow corresponding to Formula
(Figure 3-56).
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Figure 3-56: The Define screen
This will bring the user to a screen asking to Select Formula Type. At
this screen (Figure 3-57), select TRANS. Then enter the formula using the
Math, Other, Map and Function keys.
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Figure 3-57: Select Formula Type screen
EXAMPLE 1:
Divide all OD’s on the plate by 2 and multiply by 100.
Enter the formula: (OD/2)*100
This formula will be applied to the OD’s of all samples, standards, controls
and blanks that are present on the plate map.

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