Biotek PowerWave - Specifications

PowerWave Operator’s Manual 1-5
Specifications___________________________________________________
Optical Specifications
• The following specifications apply to 96-well, flat- or round-bottom plates, single
wavelength endpoint or kinetic (with intervals of at least 20 seconds) readings:
λ range: 200 to 800 nm for PowerWave 200
340 to 800 nm for PowerWave 340
λ accuracy: +/- 2 nm for PowerWave 200 and 340
λ repeatability: +/- 0.2 nm for PowerWave 200 and 340
Regular read mode end point or kinetic with interval at least 20 seconds.
Read time: 20 seconds on single wavelength
50 seconds on dual wavelength
Absorbance Measurement Range: 0-4.000 Abs (200/340 -900 nm)
Accuracy: +/- 1% +/- .010 Abs from 0 to 2.000 Abs
+/- 3% +/- .010 Abs from 2.000 to 3.000 Abs
(measured with neutral density filters
calibration test plate @ 405, 450, 490, 550, 630,
690 nm)
Linearity: +/- 1% from 0 to 2.000 Abs
+/- 3% from 2.000 Abs to 3.000 Abs (measured
@ 405 nm)
Repeatability (STD): +/- 1% +/- .005 Abs from 0 to 2.000 Abs
+/- 3 % from 2.000 Abs to 3.000 Abs (measured
@ 405 nm)
Fast mode of read or spectral scanning
When read intervals from 10 seconds to 20 seconds are selected, or spectral scanning
is performed, the specifications are as follow:
Accuracy: +/-1%+/-.010 Abs from 0 to 2.000 Abs
+/- 3% +/- 0.010 Abs from 2.000 Abs to 2.500 Abs
(measured with neutral density filters calibration
test plate @405, 450, 490, 550, 630, 690 nm)
Linearity: +/- 1% from 0 to 2.000 Abs
+/-3 % from 2.000 to 2.500 Abs (measured
@ 405 nm)