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Biotek PowerWave - Define (Method, Map, Formula and Curve)

Biotek PowerWave
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3-6 Operation
Define (Method, Map, Formula and Curve)
The DEFINE Option screen (Figure 3-5) allows the user to edit the Method,
Map, Formula or Curve Fit.
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Figure 3-5: Define Option screen
Method
The definition of a method includes selecting:
Endpoint, Kinetic or Well Scanning Read Modes
Delay first read
Incubation parameters
Wavelengths applied
Shake parameters
Kinetic analysis
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NOTE: Some screens shown here may not appear on some reader models.
READ TYPE
This option allows the user to enter which read type, Endpoint, Kinetic or
Scan.
Endpoint: The plate is read once. The unit of measure is Optical Density.
Kinetic: The plate is read several times. Three different types of data are
available:
a) For a rate determination, the data calculated is the rate of OD
change. After determining the maximum rate of change per well
using linear regression, the units are milliOD/min.
b) The R-squared value is an indication of how well the points fit the
linear curve.

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