Bruker HYPERION - Page 82

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HYPERION User Manual Bruker Optik GmbH
Operation 4
13. Take a snapshot of the video image
with aperture.
Note: In case of a manual knife-edge
aperture, it is advisable to take a snapshot
in order to be able to reconstruct the aper-
ture setting later, if required.
corresponding OPUS function
☞ See OPUS/VIDEO Manual.
14. Save the current stage position.
Note: This is only possible in case of a
motorized stage. In case of a manual
stage, skip this step.
corresponding OPUS function
☞ See OPUS/VIDEO Manual.
15. Move the stage in x- and/or y-direc-
tion until a sample-free area of the sub-
strate is in the beam path.
Note: Make sure that the detector does
not oversaturate. Do not change the set-
ting of the knife-edge aperture!
Manual x/y-stage: Rotary knobs (A
and B in fig. 3.6)
Motorized x/y-stage: Joystick and cor-
responding stage control functions in
OPUS
☞ See OPUS/VIDEO Manual.
16. Move the pinhole aperture in the
beam path. If required, correct the con-
denser setting. Note: When you see a
sharp image of a bright circle which is
aligned to the center of the cross hairs,
then the condenser is set correctly. After-
wards, move the pinhole aperture out of
the beam path again.
Thumb wheel of the pinhole aperture
for setting the condenser (A in fig. 3.9),
condenser focussing knobs (C/C’ in
fig. 3.9) and centering screws (D/D’ in
fig. 3.9)
☞ See section 4.6.
17. Activate the measuring mode. either using the IR button on the oper-
ating panel of the microscope (C in
fig. 3.4) or in the OPUS video wizard
☞ See OPUS/VIDEO Manual.
18. Start the background measurement.
Attention: Before starting the measure-
ment, open the iris aperture for setting
the Koehler illumination in transmission
(B in fig. 18) again. See step 8.
corresponding OPUS function
☞ See OPUS/VIDEO Manual.
Iris aperture for setting the Koehler illu-
mination (B in fig. 3.10)
Knurled ring underneath the condenser
Operating step Corresponding operating element(s)