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Hologic ThinPrep 5000
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ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.19
5
NON-GYNECOLOGIC SAMPLE PREPARATION
4. CytoLyt
Solution wash (page 5.12)
Add 30 mL of CytoLyt Solution to one 50-mL centrifuge tube and vortex.
Transfer the contents of this tube into the second 50-mL centrifuge tube
and vortex. The specimen is now combined into one 50-mL tube. The
empty tube can be discarded.
Centrifuge.
Pour off supernatant.
Resuspend cell pellet.
5. Evaluate cell pellet appearance (page 5.9)
If the cell pellet is not free of blood, add 30 mL of CytoLyt Solution and
repeat from step 4.
6. Add entire specimen to PreservCyt
Solution vial (page 5.10)
Allow to stand in PreservCyt Solution for 15 minutes.
7. Run on ThinPrep
5000 processor with AutoLoader using
Sequence
UroCyte
.
Fix, stain, and evaluate cytology,
OR
perform the molecular diagnostic
testing according to the manufacturer’s instructions for use.
Note:
UroCyte samples require the yellow ThinPrep UroCyte filter and
UroCyte microscope slide for processing.

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