NON-GYNECOLOGIC SAMPLE PREPARATION
5.26
ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
Techniques Used in Troubleshooting
Diluting the Sample 20 to 1
To dilute a sample suspended in PreservCyt Solution, add 1 mL of the sample that is suspended in
PreservCyt Solution to a new PreservCyt Solution vial (20 mL). This is most accurately done with a
calibrated pipette.
You may also simply count drops from an uncalibrated plastic pipette if you know how many drops
correspond to 1 mL. To calculate this, count out drops of PreservCyt Solution into a container of
known volume. When the known volume is reached, divide the number of drops by the volume (in
mL) to get the number of drops that corresponds to 1 mL. Use PreservCyt Solution rather than any
other liquid so the drop size will be consistent with the sample drops.
Glacial Acetic Acid Wash for Blood and Non-Cellular Debris
If a sample is found to be bloody during microscopic review, it can be further washed using a
solution of 9 parts CytoLyt Solution and 1 part glacial acetic acid. This should only be done after the
sample has been in PreservCyt Solution. Do not use directly with fresh specimens; nuclear
morphology may not be adequately preserved.
6. Add specimen to PreservCyt™ Solution
vial (page 5.10).
7. Run on ThinPrep
™
5000 processor with
AutoLoader using Sequence Non-Gyn. Fix,
stain, and evaluate.
8. If the new slide is sparse, call Hologic
Technical Support (page 12.1).
Problem Procedure
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