ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.25
NON-GYNECOLOGIC SAMPLE PREPARATION
Mucoid Specimens
Problem Procedure
A. Did the “Sample Is
Dilute” message appear
during processing?
1. Check to see if cellularity is adequate. If
not, use more of the pellet if available.
Prepare a slide using Sequence Non-Gyn.
B. Does the slide have an
obvious “halo” of cellular
material and/or white blood
cells.
1. Dilute the sample 20:1. Use a calibrated
pipette to add 1 mL of sample to a new
PreservCyt
™
Solution vial. Prepare slide
using Sequence Non-Gyn.
If a halo is present on the new slide, call
Hologic Technical Service (page 12.1).
C. Is the slide sparse and
does it contain mucus?
1. Pour the contents of the PreservCyt sample
vial into a centrifuge tube.
Call Hologic Technical
Service (page 12.1).
2. Concentrate by centrifugation.
— 600g for 10 min. (page 5.5) or 1200 g for 5
min.
3. Pour off supernatant and vortex to
resuspend cell pellet (page 5.7).
4. CytoLyt Solution wash (page 5.12)
5. Evaluate cell pellet appearance (page 5.9).
If pellet contains mucus, repeat from step 4.
MUCOID
ONLY
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