Sutter Instrument P-1000 - Page 8

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2.6.6 Time & Delay Modes of Cooling .............................................................................................19
2.6.7 Jaw Temperature .....................................................................................................................20
2.6.8 Edit User Notes ........................................................................................................................21
2.7 Error Messages during Pull...........................................................................................................21
3. INSTALLATION...............................................................................................................................23
3.1 Unpacking........................................................................................................................................23
3.2 Setting Up........................................................................................................................................23
3.2.1 Line Power (Mains)..................................................................................................................23
4. OPERATING INSTRUCTIONS......................................................................................................25
4.1 First Time Use ................................................................................................................................25
4.1.1 Quick Start Instructions..........................................................................................................25
4.1.2 Default Configuration..............................................................................................................33
4.2 Programs..........................................................................................................................................35
4.2.1 Program Structure...................................................................................................................35
4.2.2 Program Line Pull Cycle Parameters ....................................................................................36
4.2.3 Pull Cycle ..................................................................................................................................38
4.2.4 Start Up.....................................................................................................................................38
4.2.5 Editing a Program....................................................................................................................40
4.2.5.1 Editing Tips........................................................................................................................40
4.2.5.2 Edit User Notes .................................................................................................................40
4.3 Menu screen ....................................................................................................................................42
4.3.1 General ......................................................................................................................................42
4.4 System Options ...............................................................................................................................42
4.5 Program Options.............................................................................................................................42
4.6 Ramp Test........................................................................................................................................43
4.6.1 Heat Value Recommendations ...............................................................................................44
4.7 Pulling Pipettes...............................................................................................................................44
4.7.1 Procedures ................................................................................................................................44
4.7.2 Notes on Program Operation..................................................................................................46
4.8 Parameter Adjustment...................................................................................................................46
4.8.1 General Information ................................................................................................................46
4.8.2 Micropipette/Microinjection Needle Fabrication..................................................................46
4.8.2.1 HEAT..................................................................................................................................46
4.8.2.2 PULL Strength..................................................................................................................47
4.8.2.3 VELOCITY (Trip Point)...................................................................................................47
4.8.2.4 TIME Mode (Cooling) .......................................................................................................47
4.8.2.5 Delay Mode (Cooling)........................................................................................................48
4.8.2.6 PRESSURE Adjustment ..................................................................................................48
4.8.2.7 Filament Width..................................................................................................................48
4.8.3 Patch Pipette Fabrication .......................................................................................................48
4.8.3.1 HEAT..................................................................................................................................49
4.8.3.2 PULL Strength..................................................................................................................49
4.8.3.3 VELOCITY (Trip Point)...................................................................................................49
4.8.3.4 Cooling ................................................................................................................................49
4.8.3.5 PRESSURE........................................................................................................................50
4.8.4 Thermolock™ Pre-Heat Mode ................................................................................................50
4.8.5 Step-by-Step Patch Pipette Programming ............................................................................53
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)