Sutter Instrument P-1000 - Page 9

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4.9 Technical Tips for Pulling Micropipettes.....................................................................................54
4.9.1 Regulating the Time it takes to pull a Sharp Pipette...........................................................54
4.9.1.1 HEAT..................................................................................................................................54
4.9.1.2 Pipette Position .................................................................................................................55
4.9.2 Regulating the Length and Tip Size of a Sharp Pipette ......................................................55
4.9.2.1 HEAT..................................................................................................................................55
4.9.2.2 Filament Width..................................................................................................................55
4.9.2.3 Air Flow ..............................................................................................................................55
4.9.2.4 DELAY Mode of Active Cooling.......................................................................................55
4.10 Fire Polishing ................................................................................................................................56
4.11 Heating Filaments ........................................................................................................................57
4.11.1 General Information..............................................................................................................57
4.11.2 Filament Positioning and Aligning ......................................................................................57
4.11.3 Box Filament ..........................................................................................................................58
4.11.3.1 Positioning .......................................................................................................................58
4.11.3.2 Geometry..........................................................................................................................59
4.11.4 Trough Filament ....................................................................................................................59
4.11.4.1 Positioning .......................................................................................................................60
4.11.4.2 Geometry..........................................................................................................................60
5. MAINTENANCE...............................................................................................................................61
5.1 Cleaning ...........................................................................................................................................61
5.2 Heating Filament Replacement ....................................................................................................61
5.2.1 Filament Replacement (Step by Step) ...................................................................................61
5.2.2 Air Jet Position.........................................................................................................................61
5.2.3 Positioning the Filament in Relation to the Glass Capillary ..............................................61
5.3 Pulley Adjustment ..........................................................................................................................63
5.4 Regeneration of Drierite Granules................................................................................................64
5.4.1 Removing the Canister ............................................................................................................64
5.4.2 Replacing the Granules ...........................................................................................................64
5.4.3 Reinstalling the Canister.........................................................................................................65
6. TROUBLESHOOTING ....................................................................................................................67
6.1 Controlling Pipette Tip Shapes .....................................................................................................67
6.1.1 Problem: What type of glass should be used? .......................................................................67
6.1.2 Problem: The resistance of pulled pipettes is too low. How can a higher-resistance
pipette be pulled?...............................................................................................................................67
6.1.3 Problem: How can tips be made even smaller?.....................................................................67
6.1.4 Problem: How can the size of a patch-pipette be increased?...............................................68
6.1.5 Problem: Patch-pipette tips vary in size from pull to pull...................................................68
6.1.6 Problem: Difficulty making an injection pipette with a 0.5 – 1 mm tip that also has a
very short final taper and tip (20 to 50 mm long). How can this be done? .................................68
6.1.7 Problem: The electrodes are bent. How can they be made to pull straight? .....................68
6.1.8 Problem: One electrode is much longer than the other electrode. .....................................68
6.1.9 Problem: The shape and resistance of the pipette changes from pull to pull....................69
6.2 System Operation/Function Problems .........................................................................................69
6.2.1 Startup Problems .....................................................................................................................70
6.2.2 General Operational Problems ...............................................................................................70
6.2.3 On-Screen Emergency Reset Messages .................................................................................70
P-1000 FLAMING/BROWN MICROPIPETTE PULLER SYSTEM OPERATION MANUAL – REV. 3.02 (20161118)