Thermo Spectronic BioMate 3 User Manual

Troubleshooting Guide
7. Calibrate the grating
a. Move the grating to 10 steps below the peak.
b. Scan to 10 steps above the peak.
c. Find the actual energy peak in the scan.
d. On a VIS instrument, repeat steps 7.a – 7.c for 4 wavelengths
i. 494.0nm peak
ii. 585.2nm valley
iii. 775.0nm peak
iv. 807.1nm valley
e. On the UV instrument, repeat steps 7.a – 7.c for 4 wavelengths
i. 824.1nm peak
ii. 882.7nm peak
iii. 895.8nm peak
iv. 905.2nm peak
v. 917.0nm peak
f. Set the final calibration by the average of the errors from the calibration peaks.
8. On the VIS instrument, move to 681nm and determine the ADC integration time.
9. Move to the last wavelength used on the walkup (ATC) screen, and zero the system.
Spectronic GENESYS 10 UVscanning, Spectronic GENESYS 10 > Revision 1.103, Spectronic
BioMate 3 > Revision 1.001 and Spectronic GENESYS 10 Bio > Revision 1.000 Initialization
1. Power on.
2. Calibrate turret
a. Rotate the turret while reading the turret sensor.
b. After we have detected 3 small openings without finding the big opening, reverse the
turret rotation direction.
c. After we have detected 6 more small openings without finding the big opening, return a
turret calibration failure error.
d. When we find the big opening (indicates position B), center the turret in the opening.
3. Initialize the grating
a. Moving to the grating calibration switch.
b. On the VIS instrument, move to grating step 8695 (ideally 1095nm).
c. On the Spectronic GENESYS 10 UVscanning, move to grating step 15948 (ideally
716nm).
d. On other UV instruments, move to grating step 6475 (ideally 850nm).
4. Initialize the filter wheel
a. Rotate the filter wheel while recording energy levels.
b. Look for an area that passes light.
c. Center the filter wheel in the position found.
5. Find zero order
a. Spectronic GENESYS 10 UVscanning.
i. Scan between grating steps 1472 and 2432 for the start of zero order saturation.
ii. Scan towards grating step 3968 for the end of zero order saturation.
b. Spectronic GENESYS 10, Spectronic BioMate 3 and Spectronic GENESYS 10 Bio.
i. Scan between grating steps 555 and 2035 for the start of zero order saturation.
ii. Scan towards grating step 2405 for the end of zero order saturation.
c. Set the initial calibration to the center of the zero order area.
3-2

Thermo Spectronic BioMate 3 Specifications

General

Brand	Thermo
Model	Spectronic BioMate 3
Category	Measuring Instruments
Language	English