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Thermo Spectronic BioMate 3 User Manual

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Troubleshooting Guide
5. Find zero order by
a. Scan between grating steps 1472 and 2432 for the start of zero order saturation.
b. Scan towards grating step 3968 for the end of zero order saturation.
c. Set the initial calibration to the center of the zero order area.
6. Finish calibrating the filter wheel
a. Move the grating to 0nm.
b. Rotate the filter wheel while recording energy levels.
c. Look for the end of the large dark area to locate the filter wheel.
d. Center the filter wheel on the next position, the open position.
7. Initialize the calibration gains
a. Select the appropriate filter for 224nm.
b. Scan from 224nm to 235nm.
c. Zero the instrument at the grating step with the highest energy level from the above scan.
8. Calibrate the grating
a. Scan from 242nm to 253nm looking for the peak at 247.59nm.
b. Scan from 523nm to 534nm looking for the peak at 529.02nm.
c. Scan from 819nm to 830nm looking for the peak at 824.09nm.
9. Move to the last wavelength used on the walkup (ATC) screen, and zero the system.
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Thermo Spectronic BioMate 3 Specifications

General IconGeneral
BrandThermo
ModelSpectronic BioMate 3
CategoryMeasuring Instruments
LanguageEnglish

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