OPERATION
Carl Zeiss Illumination and contrast procedures Axio Examiner
60 M60-2-0003 e 05/2012
• For aperture stop setting (contrast), remove one eyepiece from the tube socket, and look into the
socket with a naked eye. Set the aperture stop using the adjusting lever (Figure 4-2/6) to approx.
2/3 ... 4/5 of the objective output pupils' diameter (Figure 4-2/E). This aperture stop setting provides
the best contrast with almost complete resolution in most applications, and thus constitutes the best
compromise for the human eye.
• Insert the eyepiece into the tube socket again.
Every objective exchange changes both the visual field size and the objective aperture. This
requires a new setting of bright field and aperture stop in order to achieve optimum results.
If objectives <
10x are used, the aperture stop needs to be opened completely. To achieve a
better contrast for such big visual fields, the luminous-
field diaphragm may also be used,
reducing its opening up and into the visual field.
(4) VIS/IR toggling
To obtain a higher contrast with specimens presenting high scattering within the tissue, it is
recommended to use infrared light. To change over, proceed as follows:
• Set the control wheel (Figure 1-3/17 and Figure 1-4/18) at the stand from visual (white circle) to
infrared (red circle).
• If required, use a Senarmont polarizer and analyzer modules for IR (see sections 3.1.5 and 3.3.7).
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