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Zeiss Axio Examiner - Page 64

Zeiss Axio Examiner
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OPERATION
Carl Zeiss Illumination and contrast procedures Axio Examiner
64 M60-2-0003 e 05/2012
Re-adjust the knurled screw at the DIC slider (Figure 4-3/2), adjusting the diagonal black strip to the
center of the visual field.
Remove the auxiliary microscope, and insert the eyepiece again.
(5) Setting transmitted light DIC
Put an objective suitable for DIC into work position using the shift knob (Figure 4-5/7).
Swing in the analyzer module at the reflector turret (Figure 4-5/8).
Swing in a suitable DIC prism I, II or III (number as shown at the condenser revolving disk).
The condenser used must not be equipped with DIC prisms which are installed in combination with
polarizing filters.
Switch on the SÉNARMONT polarizer (Figure 4-5/2)(polarizer and λ/4 plate) in the lock-in position.
Set the luminous-field diaphragm (Figure 4-5/8) and the aperture stop (Figure 4-5/6) according to
KÖHLER's rules.
To adjust the optimum contrast for SÉNARMONT DIC, turn the polarizer using the thumb wheel
(Figure 4-5/3) of the SÉNARMONT polarizer. The λ/4 plate located above needs to be switched on at
all times to ensure that contrasting works according to SÉNARMONT. If the DIC slider is in the proper
center position, darkness is achieved in the SÉNARMONT polarizer lock-in position, i.e., the path
difference is 0 nm in this position.

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