Abbott CELL-DYN 3200 - WBC Flagging; RBC;PLT Measurement

CELL-DYN
®
3200 System Operator’s Manual
3-19
9140181H—October 2001
Section 3 Principles of Operation
The WBC subpopulations are further identified by the following colors:
Neutrophils — yellow
Lymphocytes — blue
Monocytes — purple
Eosinophils — green
Basophils — white
NOTE: The basophils are displayed as white dots but appear as black dots on
color printouts.
The WBC scatter information is usually displayed in two scatterplots as shown in
Figure 3.8:
SIZE/COMPLEXITY The size (0° scatter) information is plotted on the
Y axis and the complexity (10° scatter) information
is plotted on the X axis.
GRANLRTY/LOBULARITY The granularity (90°D scatter) information is plotted
on the Y axis and the lobularity (90° scatter)
information is plotted on the X axis.
WBC Flagging
Refer to the “Operational Messages and Data Flagging” subsection of this section
for WBC flagging information.
RBC/PLT Measurement
Overview
The Optical Channel is used for the determination of RBC and PLT data. During
sample aspiration, 1.67 µL of sample is segmented in the Shear Valve for RBC/PLT
measurement.
The Diluent/Sheath Syringe dispenses 2.79 mL of diluent to the Shear Valve. The
sample and diluent are then transferred to the RBC/PLT Mixing Chamber where
the dilution is swirl mixed, resulting in a 1:1,675 dilution ratio.
The Sample Transfer Pump transfers the RBC/PLT dilution from the mixing
chamber to the sheath feed nozzle in the Optical Flow Cell. The Sample Metering
Syringe injects 24 µL of RBC/PLT dilution into the sheath stream. The sample
stream is then hydrodynamically focused to align the cells in single file as they pass
through the Optical Flow Cell, which is an optically clear quartz chamber. A
vertically polarized Helium Neon Laser is the light source.
There are 256 size channels for each of the parameters, each RBC size channel
being equivalent to 1 fL and each PLT size channel being equivalent to 0.137 fL.
The RBC parameters are calculated using 0°, 10°, and 90° sensor data, while the
PLT parameters are calculated using 0° and 10° sensor data.