Abbott CELL-DYN 3200 User Manual

6-8
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Calibration Procedures
Pre-Calibration Procedures
Section 6
10. Confirm that the waste container is no more than half full. If necessary, empty
it as described in Section 8: Hazards; Subsection: Handling and Disposing
of Biohazardous Materials.
11. Confirm that background counts are within limits. If the system has been idle
for fifteen minutes or more, a Background count should be run immediately
prior to running any calibration specimens.
12. Confirm that the Operator ID number is entered.
Calibration Materials
Calibrator Requirements
• For commercial calibrators, follow the directions given in the package insert.
Be certain to carefully read and follow directions given for warming and
mixing.
• Auto-Cal Method: The calibrator should be cycled for a minimum of 6 and a
maximum of 10 consecutive runs when calibrating in either Open or Closed
Mode.
• Enter Factor Method: The calibrator should be cycled for a minimum of 6
consecutive runs. Additional samples and/or repetitions of the specimens
may be used to achieve calibration accuracy beyond NCCLS
recommendations.
• The Calibrator sample should contain a minimum of 5.0 mL. If necessary,
aliquot a sufficient amount of sample into a single tube for Closed Mode
processing.
Fresh Whole Blood Requirements
The International Committee for Standardization in Hematology (ICSH) defines a
fresh blood sample as one available for processing less than four hours following
venous sampling.
Normal Sample
• Blood samples should be from the general patient population, with values for
all parameters which are within the laboratory’s normal range.
• All cellular morphology must be normal.
• No known interfering substances should be present (for example, lipemia,
icterus, medications).
• All samples must be properly collected in the EDTA anticoagulant used by
the laboratory.
• Each tube should contain at least 90% of the nominal collection volume of
blood.
• Samples should be at room temperature and mixed properly.

Abbott CELL-DYN 3200 Specifications

General

Brand	Abbott
Model	CELL-DYN 3200
Category	Measuring Instruments
Language	English