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CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Calibration Procedures
Enter Factor Method
Section 6
Confirming Closed Mode Calibration
1. Press [RUN] followed by [SPECIMEN TYPE]. Select an empty QC file and press
[QC SPECIMEN].
2. Refer to Worksheet 7, Calibration Confirmation, in subsection Manual
Calibration Worksheets. Make copies of this worksheet as necessary.
3a. Obtain the calibrator used in the calibration process and run it three times into
the empty QC file. When the runs are completed, print the appropriate QC
file and transfer the mean values to the appropriate spaces on Worksheet 7.
3b. Take each of the five samples used in the calibration process and run each
sample once. When done, print the appropriate QC file and transfer the values
in the appropriate space on the Worksheet 7.
NOTE: Closed Mode Calibration may also be confirmed by running
commercial controls.
4. Follow the instructions on the worksheet for comparing the mean value
against the assay value (calibrator) or reference mean (fresh whole blood).
5. If the difference between the mean and assay value/reference mean is within
the stated tolerance limits, then calibration has been confirmed for that
parameter. Proceed to calibrate the Open Mode if not already calibrated.
6. If a parameter’s mean is outside the stated tolerance limits, repeat steps 3 and
4 above for that parameter. If the mean is still outside the stated tolerance
limits, call the Abbott Hematology Customer Support center for assistance
(at 1-800-CELL-DYN in the U.S.).
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