6-32
CELL-DYN
®
3200 System Operator’s Manual
9140181K—July 2002
Calibration Procedures
Auto-Cal Method
Section 6
NOTE: Because Factor % Diff values highlighted in purple can be changed
to green using the Enter key, it is also possible for the operator to
overwrite these values. However, the change only appears on the
screen; the actual values calculated by the calibration program
remain intact and the Mean Factors are not affected.
c. If no component was changed and your calculations are correct, DO
NOT CALIBRATE. CONFIRM THAT ALL PRE-CALIBRATION
PROCEDURES WERE COMPLETED AND THEN CALL THE
ABBOTT HEMATOLOGY CUSTOMER SUPPORT CENTER FOR
ASSISTANCE (at 1-800-CELL-DYN in the U.S.).
Completing Calibration Log
1. Complete the Calibration Log (adding comments) as required. If a printout of
the log is desired, press [PRINT LOG].
2. Press [RETURN] to return to the mainCALIBRATION screen.
3. Press [PRINT] to obtain a copy of the new Closed Sampler Calibration Factors.
Confirming Closed Mode Calibration
To confirm calibration in the Closed Mode, follow the instructions below. Use the
same Calibrator or Fresh Whole Blood Sample that was used to calibrate the
instrument.
1. In the CALIBRATION menu, press [MAIN] to return to the MAIN MENU.
2. In the MAIN MENU, press [RUN] followed by [SPECIMEN TYPE]. Select an
empty QC file and press [QC SPECIMEN].
3. Refer to Worksheet 7, Calibration Confirmation, in Manual Calibration
Worksheets at the end of this section. Make copies of this worksheet as
necessary.
4. Run the same Calibrator or Fresh Whole Blood samples in duplicate.
NOTE: You may also elect to Run Commercial Control as a means of
confirming calibration.
5. After each run, observe the values displayed on the RUN screen for those
parameters that were calibrated and that now need confirmation. Write these
values in the appropriate space on Worksheet 7.
6. Follow the instructions on this worksheet for calculating a mean and
comparing the mean value against the reference value.
7. If the difference between the mean and reference (assay) value is within the
stated tolerance limits, then calibration has been confirmed for that
parameter. Go to step 9 below.
8. If a parameter mean is outside the tolerance limits, perform steps 4 and 5
again. If the mean is still outside the tolerance limits, call the Abbott
Hematology Customer Support Center for assistance (at 1-800-CELL-DYN
in the U.S.).
9. When Closed Mode calibration has been confirmed, press [MAIN] to return to
the MAIN MENU.
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