CELL-DYN
®
3200 System Operator’s Manual
11-25
9140181H—October 2001
Section 11 Quality Control
If all efforts fail to bring results within acceptable limits, contact the Abbott
Hematology Customer Support Center (at 1-800-CELL-DYN in the U.S.) for
assistance. Customers outside the U.S. contact your local Hematology Customer
Support Center.
Interpreting X-B RBC Results
A suggested protocol and guidelines for interpreting X-B data can be found in
Chapter 1 of Laboratory Hematology: An Account of Laboratory Techniques,
edited by I. Chanarin.
8
X-B Analysis for WBC
Overview
Because the WBC Differential parameters have a wide dynamic range, it is difficult
to use a moving average algorithm to control these results. Therefore, the
CELL-DYN 3200 System uses data obtained from the MAPSS
TM
technology used
for the WBC Differential measurement.
Five main subpopulations of WBCs, which can vary widely in absolute number
and percentage values, are identified by the CELL-DYN 3200 System. Even
though these parameters have varying dynamic ranges, they maintain a relatively
constant modal position on each axis of the scatterplots. It is expected that these
optical characteristics of the WBC Differential subpopulations will remain stable
over time without impact from the wide dynamic ranges of the individual
parameters. This constant modal position, which is sensitive to changes in the
instrument’s optical measurement process, can be monitored by the instrument and
used to control the WBC Differential parameters in much the same way that the
RBC indices are used to control the RBC parameters.
The CELL-DYN 3200 System monitors the modal positions of the lymphocyte and
neutrophil clusters on each axis of the 0°/10° scatterplot. It also monitors the modal
positions of the neutrophil cluster on each axis and the angle of the neutrophil/
eosinophil separation on the 90°/90° depolarized scatterplot. These seven
measurements are then averaged for each batch of 20 patients using a moving
average calculation similar to that developed by Dr. Bull for the RBC indices. For
convenience, this process is called X-B WBC.
Table 11.1 Troubleshooting X-B RBC
X-B
Pattern
If the MCV If the RBC If the HGB
Index
Derivation
is
increased
is
decreased
is
increased
is
decreased
is
increased
is
decreased
MCV
will be
High Low N/A N/A N/A N/A MCV
MCH
will be
N/A N/A Low High High Low HGB/RBC
MCHC
will be
Low High Low High High Low HGB/HCT
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