14-62
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Reticulocyte Package
Routine Operation
Section 14
Procedure: Quality Control
1. Warm the control material according to the manufacturer’s
recommendations. See Quality Control, Mixing and Handling within this
chapter.
2. Use reticulocyte reagent and verify the expiration date. Store the stock
reagent in the dark at room temperature.
3. Label one tube of reticulocyte reagent for each level of control material.
4. Pipette 20 µL of the control material into each labeled tube of reticulocyte
reagent.
5. Incubate the prepared control specimens for 15 minutes, on the rotator or in
a rack, after fully inverting the stained specimens 5 times. Incubation is
performed at room temperature according to the reagent package insert.
NOTE: The stained control specimens may incubate for up to 30 minutes
prior to processing on the CELL-DYN 3200 System.
6. Verify that the Reticulocyte Package is turned ON. For instructions on
turning ON the Reticulocyte Package, refer to Retic Menu Options, Turning
the Reticulocyte Package ON and OFF within this chapter.
7. From the RETIC MAIN menu screen press the [RETIC RUN] key.
8. From the RETIC SPECIMEN TYPE screen, move the cursor to the desired QC
file and press the [QC SPECIMEN] key. The RETIC RUN RESULT screen for QC
specimens is displayed. The control file information is located in the upper
left-hand corner of the screen.
9. Open the well-mixed, prepared control specimen tube and immerse the Open
Sample Aspiration Probe in the sample.
10. Press the Touch Plate located behind the probe to start the cycle. The BUSY
indicator light on the Analyzer Status Indicator Panel will be illuminated in
yellow. The Status Box on the RETIC RUN RESULT screen will display
messages to indicate the various stages of the cycle.
11. Remove the tube when the beep sounds. The Wash Block moves down the
probe and cleans it.
12. When the cycle is completed, the Wash Block moves up the probe and the
word READY displays in the status box.
13. Repeat steps 8 through 12 for all prepared control specimens.
14. Verify that the control results are acceptable.
NOTE: Out-of-range results are displayed in color. Data invalidating alerts,
such as Fragile RBCs, are not valid when running commercial
controls.
15. If the results are unacceptable, repeat the run. If the results are still
unacceptable, run the other levels of the control material. If the results are
still unacceptable, prepare another stained dilution of that level of the control
material. If the results on all levels are unacceptable, troubleshoot
accordingly. See Section 10: Troubleshooting and Diagnostics.
16. When the control results are acceptable, patient samples may be analyzed.
To Next Page
Loading...
