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Running an Isocratic Analysis
Entering Settings
Entering Settings
To set up the isocratic analysis you will set the LC system settings to default and
then modify selected settings, the other settings will remain with their default
values. You will then save these settings to a method called ISO.
1 Enter the Analysis screen.
2 Set the vial range as 1 to 1.
3 Set the number of injections to 1.
4 Press Enter to skip the method name section.
5 Select the Settings button [F1].
6 Select LC System from the menu.
7 Select the Default button [F7] and select the Yes button to load defaults.
8 Press the Esc key.
9 Select the Settings button [F1].
10 Select the pump from the menu (either ISO, BIN or QUAT).
11 Press the Pressure button [F3].
12 Enter 400 as the upper pressure limit.
13 Select the Done button [F6].
14 Enter the following values: %B 65, (%C OFF, %D OFF for quarternary pump. If you
have a binary pump, set %B to 65.).
15 Set the Flow to 1,5 ml/min.
16 Confirm by selecting “Done” to enter the Analysis screen
17 Enter the values shown in Table 2 in the Analysis screen.
NOTE The channels of a pumping system are named A, B, C and D (depending on the pump
type). %A is automatically calculated by 100% - (%B + %C + %D). If no values for
%B, %C and %D are entered, %A is always 100%.
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