Agilent Technologies 1100 Series User Guide

83
Using the Manual Injection Valve
Completely Filling the Sample Loop
Completely Filling the Sample Loop
This is the conventional method in which an excess of sample is used to fill the
sample loop completely. The volume of the loop determines the injection volume.
1 Fill syringe with sample.
2 Move valve to LOAD position.
3 Insert needle of syringe into needle port until needle touches stator face. Do not press
too hard.
4 Slowly inject sample.
5 Leave syringe in position and move valve to INJECT position.
6 Remove syringe.
Partially Filling the Sample Loop
Use this method when only small quantities of sample are available. In this method
the syringe determines the injection volume.
1 With valve in INJECT position, use needle port cleaner to flush needle port with
about 1 ml of mobile phase (this will reduce residual contamination from previous
injection).
2 Move valve to LOAD position.
3 Fill syringe with required volume of sample (not more than half of loop volume).
4 Insert needle of syringe into needle port until needle touches stator face. Do not press
too hard.

Agilent Technologies 1100 Series Specifications

General

Brand	Agilent Technologies
Model	1100 Series
Category	Laboratory Equipment
Language	English