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Running an Injector Program
Creating an Injector Program
Creating an Injector Program
The injector program is part of the method. The injector program screen can be
accessed using the Inj. Program button [F3] in the Autosampler Settings screen.
This section describes an injector program to do sample preparation involving a
precolumn derivatization reaction. This is required when the analytes lack
chromophores and in their original chemical structure cannot be detected with the
required sensitivity.
To create this injector program you will:
• set the LC system to the default settings
• modify the injector program settings in the Inj. Program screen, the other settings
will remain with their default values, and
• save the method with the name DERIV.
When the method called DERIV is started, the program is executed followed by the
analytical run.
The first step of the program involves drawing derivatization reagent into the needle
capillary, followed by sample and then reagent again. To ensure the reagent and
sample react efficiently, the plunger in the metering device of the autosampler is
moved back and forth. This mixes the sample and reagent as they travel up and
down the capillary. Following mixing, the derivatization requires a pause to allow
the sample and reagent to fully react, before the derivatized sample is injected onto
the column.
Entering Injector Program Settings
1 Enter the Analysis screen.
2 Select the Settings button [F1].
3 Select LC System from the menu.
4 Select the Default button [F6] and select the Yes button to load defaults.
5 Press Esc to go back to the Analysis menu.
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