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Working with the Control Module

Automating Analyses

You can edit an existing timetable line by pressing the Enter key. Use the Delete

button [F6] and make a choice from the pop-up menu to delete either the selected

line or the whole timetable.

You can copy and paste timetable lines by selecting the respective choices available

from the context menu (m key).

Automating Analyses

You can use the Sequence screen to create completely automatic unattended

analyses from sample preparation to injection. The Sequence screen is accessed by

using the Sequence button [F4] in the Analysis or Samples screen.

Using the Sequence screen you can link several methods together. For example, you

can first run a method containing an injector program to do sample preparation

followed by an analytical run to analyze a batch of samples. You can then run a

second method to analyze further samples with different analytical conditions. A

delay time can be set in the sequence line. When the second method is loaded, it

waits for a specified time before starting the analysis, allowing the column to

equilibrate to the new conditions. All sequence events can be traced in the Sequence

Logbook available through the Logbook button [F5] in the Sequence screen.

At the end of the sequence you can specify either to load a method (e.g. to flush the

LC system to remove buffer salts to avoid crystallization or to program a soft shut-

down method) or to turn off the LC system using the End Actions button [F1]. If

both options are selected, the shut-down method will be loaded to be available for

the next user. However, it will not be executed before the turning off.

You can set up automatic recalibrations using the Calibration Settings screen. This

screen allows you to attach calibration settings to a sequence line. The Calibration

Settings screen is accessed by selecting the Calibration button in the Sequence

screen.

You can recalibrate using one or more standards and have the flexibility of choosing

various calibration intervals and patterns. You can define within a sequence line the

frequency to recalibrate and the order of calibration vial analysis using the Alter and

Multi settings. Alter analyzes the calibration vials alternately. Multi analyzes the

calibration vial or vials in complete groups according to the calibration interval.

A sequence line consists of the following:

Agilent Technologies 1100 Series User Guide

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