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Using the Degasser
Changing Solvents
Changing Solvents
If you are changing to a solvent that is immiscible with the solvent currently in the
tubing:
1 Replace the current solvent:
with iso-propanol, if current solvent is organic, or
with water, if current solvent is an organic buffer or salt.
2 Flush the online degasser and all tubing thoroughly.
3 Replace the iso-propanol or water with the new solvent.