110
Running a Gradient Analysis
What You Will Need
What You Will Need
Instruments Agilent 1100 Series binary or quaternary pump, autosampler,
UV-detector and degasser.
Column A 125 mm × 4.0 mm Hypersil ODS, 5 µm (Agilent
Technologies part number 7982618-564).
Solvents LC-grade bidistilled water and acetonitrile.
Sample The Agilent Technologies isocratic standard sample (Agilent
Technologies part number 01080-68704). This contains
0.15 wt.% dimethylphthalate, 0.15 wt% diethylphthalate,
0.01 wt.% biphenyl and 0.03 wt.% o-terphenyl dissolved in
methanol.